Figure 1.

Schematic illustration of experimental design. Mononuclear cells (red arrow) were isolated by Ficoll gradient centrifugation from peripheral blood sample and were (A) stained with CD14 and CD16 antibodies for flow cytometric analysis and (B) cultivated for pharmacological testing of CTRP3, histological H&E staining and immunostaining for CD14 and CD16 (scale bar, 5 or 20 µm). PE, phocoerythrin; APC, allophycocyanine; Cy, cyanine; FSC, forward scatter; SSC, side scatter; Q, quadrant; RT-PCR, reverse transcription PCR; CTRP3, complement C1q tumor necrosis factor-related protein-3.