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. 2020 Feb 17;16:17. doi: 10.1186/s13007-020-00564-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Validation of primers for biomass quantification of tea plant and pathogen. a Amplification results for three genes using C. camelliae (C.c)-infected tea plant DNA. DNA. M: DL2000 Marker, 1: Cs18SrDNA1, 2: GAPDH, 3: ITS. b–d Melting curve analyses of three genes. Each qRT-PCR product had a single melt curve indicating the breakdown of only one PCR product. b Cs18SrDNA1, c GAPDH, d ITS. e–g The primer efficiency for the PCR quantification of the gene was determined using a serial dilution of DNA templates from tea plant, C. camelliae (C.c), and C. camelliae-infected tea plant (tea + C.c). e Cs18SrDNA1, f GAPDH, g ITS. The respective correlation coefficients (R²) are indicated