Fig. 15.

IOs and MF modulate RUNX-1 and RANKL in osteoclasts. Representative photographs from immunofluorescence TRAP staining (A) supported by qRT-PCR (B) data. Additionally, immunofluorescence staining for Cathepsin K (D) was performed and support with western blot analysis (E). As revealed by western blots, IOs and MF reduce amount of RUNX-1. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the results between corresponding bars representing MF− and MF+ groups, and as number sign (#) when comparing to CTRL MF−. *p < 0.05, ##, **p < 0.01, ***, ###p < 0.001