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. 2020 Jan 28;19(3):2202–2210. doi: 10.3892/etm.2020.8475

Figure 1.

Figure 1.

Pyroptotic cell death is induced by LPS in cultured dental pulp cells. (A) Human dental pulp cell surface marker expression was examined using flow cytometry. (B) Effects of different concentrations of LPS (0, 10, 100, 200, 300, 500 and 1,000 µg/l) on human dental pulp cell viability. (C) Human dental pulp cell viability was inhibited by LPS as determined by sulforhodamine B assay. (D and E) The protein levels of (D) IL-1β and (E) caspase-1 were normalized to that of β-tubulin. (F and G) The protein levels of (F) (pro)-IL-1β and (G) (pro)-caspase-1 were normalized to that of β-tubulin. (H) Western blot assay was used to detect the contents of IL-1β, caspase-1, pro-IL-1β and pro-caspase-1. (I) IL-18 contents were determined by ELISA. *P<0.05 and **P<0.01 vs. control; ##P<0.01 vs. 48 h. LPS, lipopolysaccharide; IL, interleukin.