Table I.
Sequences of the primers used for reverse transcription-quantitative PCR.
| Gene name | Forward primer | Reverse primer |
|---|---|---|
| ATGL | CACTTTAGCTCCAAGGATGA | TGGTTCAGTAGGCCATTCCT |
| HSL | GGTGACACTCGCAGAAGACAATA | GCCGCCGTGCTGTCTCT |
| PDK4 | CACAGTTGAGCACCAAGA | AGCCTGACATAGAGTAGAGA |
| CPT1a | CCACTGATGAAGGAAGGAG | TAGTAGTTGCTGTTGACCAT |
| CPT1b | CTCCACAAGCCAGATTCC | ACTCACCGTCTCAGAACT |
| FASN | CTTGTCCAGGTTCGTGAG | TGTTGCTTCGGTGATGAG |
| SCD1 | CTTCACCACATTCTTCATCG | TCCCGTCTCCAGTTCTTT |
| DGAT1 | GGCATCATACTCCATCATCT | TCTCGGTAGTTCAGATTGTC |
| PPARα | GAGAAAGCAAAACTGAAAGCAGAGA | GAAGGGCGGGTTATTGCTG |
| PPARGC1A | TGGATGAAGACGGATTGC | GCGACTGTGGTTGTGTAT |
| GAPDH | AACAGGGTGGTGGACCTCAT | TGCTCTCAGTATCCTTGCTG |
ATGL, adipose triglyceride lipase; HSL, lipase E, hormone sensitive type; PDK4, pyruvate dehydrogenase kinase 4; CPT1a/b, carnitine palmitoyltransferase 1A/B; FASN, fatty acid synthase; SCD1, stearoyl-CoA desaturase; DGAT1, diacylglycerol O-acyltransferase 1; PPARα, peroxisome proliferator activated receptor α; PPARGC1A, PPARG coactivator 1 α.