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. 2020 Feb 11;12:15. doi: 10.3389/fnagi.2020.00015

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Copyright © 2020 Piotrowska, Winter, Carare and Bechmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the spread of the injected tracer in living and sacrificed rats at 30 min and 90 min after tracer injection. Images show maximum projections of horizontal sections (a–d) which were reconstructed coronally (a’–d’) of one animal per group. Evidently, the tracer reaches the contralateral hemisphere, spreads along the vasculature, fiber tracts and disseminates further within both hemispheres in living animals, while it diffuses mostly along ipsilateral hippocampal fiber tracts surrounding the ventricles in non-vital brains.