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. 2020 Feb 11;12:15. doi: 10.3389/fnagi.2020.00015

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Copyright © 2020 Piotrowska, Winter, Carare and Bechmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deep cervical lymph nodes (DCLN) of rats 90 min post injection. Autofluorescent material was eliminated by subtracting the red channel from the pictures. In DCLN (a,b) of a living rat tracer is found within cells along the medullary trabecula, while no tracer could be detected within any dissected lymph node of sacrificed rats (c,d). Box plot shows numbers of segmented voxels depicting fluorescent tracer within LN groups (e). Tracer amounts in living compared to sacrificed animals 90 min post injection are significantly higher in right and left DCLN as well as SCLN (all ***p ≤ 0.001).