Figure 3.

Effect of PDJ on LPS-induced production of the inflammatory mediators NO and PGE₂ in RAW264.7 cells. RAW264.7 cells were pretreated with LPS (1 µg/ml) at 37°C for 1 h and then subsequently treated with PDJ (0, 2.5 or 5 µg/ml) for 24 h. (A) NO production. (B) PGE₂ production. Data are presented as the mean ± SEM of three independent experiments. ^###P<0.001 vs. untreated group; **P<0.005 vs. LPS-only treated group; ***P<0.001 vs. LPS-only treated group. PDJ, polyphenolic extract from the Dowijigi flower; LPS, lipopolysaccharide; NO, nitric oxide; PGE₂, prostaglandin E₂.