Figure 5.

Effect of PDJ on the LPS-induced mRNA expression of COX2 and iNOS in RAW264.7 cells. RAW264.7 cells were pretreated with LPS (1 µg/ml) at 37°C for 1 h and then subsequently treated with PDJ (0, 2.5 or 5 µg/ml) at 37°C for 24 h. mRNA expression levels of (A) COX2 and (B) iNOS were measured by reverse transcription-quantitative PCR. Data are presented as the mean ± SEM of three independent experiments. ^###P<0.001 vs. untreated group; ***P<0.001 vs. LPS-only treated group. PDJ, polyphenolic extract from the Dowijigi flower; LPS, lipopolysaccharide; iNOS, inducible nitric oxide synthase; COX2, cyclooxygenase-2.