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RT-PCR validation of ECM-related genes following oral shilajit supplementation. Expression levels of selected genes identified by IPA were independently verified using quantitative real-time PCR. S125 represents shilajit 125 mg and S250 represents shilajit 250 mg. Data are mean ± SEM (n = 10–13). *p < 0.05 compared to visit 2. †p < 0.05 compared to placebo.
