Table 1.

Diagnostic findings in 16 patients with antilaminin‐332 mucous membrane pemphigoid

Sample	Sex	Age (years)	DIF (BMZ)	SSS dermal binding	IB Ln‐332	IP Ln‐332	ELISA		IIF (KO skin)		Cancer
							Ln‐332	Col7	Col7	Ln‐332
1	M	54	IgG 4+, C3c 3+, IgA 2+, IgM 2+	IgG 3+, IgA+	Neg	np	np	−	+	−	Yes
2	F	59	Neg	IgG 2+	α3, β3	np	np	−	+	−	No
3	M	65	IgG 3+, C3c 2+, IgA 1+	IgG 1+	α3	np	np	np	np	np	No
4	F	86	IgG 3+, C3c 3+, IgA 2+, IgM ±	IgG 1+	γ2	np	np	−	+	−	No
5a	M	27	IgG 2+, C3c 3+	IgG 2+	α3	np	+	−	np	np	No
6a	M	25	IgG 3+,C3c 3+	IgG 2+	α3	np	+	−	np	np	No
7a	F	65	IgG 3+, C3c +/2+	IgG 2+	α3	np	+	np	np	np	No
8a	F	42	IgG 3+, C3c 2+	IgG 2+	α3	np	+	−	np	np	No
9a	M	70	IgG 2+	IgG 1+	α3	+	np	np	np	np	Yes
10	M	82	IgG 3+, C3c 3+	IgG 3+	α3, β3, γ2	np	np	−	np	np	No
11	M	77	np	IgG 2+	α3, β3	np	np	−	+	−	na
12b	F	52	IgG 3+, C3c 3+, IgA 1+	IgG 2+	Neg	np	np	−	+	−	No
13	M	81	np	IgG 3+	α3	np	np	np	np	np	na
14a	M	56	IgG 2+, IgA 2+	IgG dubious	α3	np	+	np	np	np	Yes
15	M	63	IgG 3+, C3c 2+	IgG 1+	α3	np	np	np	np	np	na
16a , c	F	64	IgG 3+/4+, C3c +/2+	IgG 3+	α3	−	+	np	np	np	No

BMZ, basement membrane zone; Col7, type VII collagen; DIF, direct immunofluorescence; ELISA, enzyme‐linked immunosorbent assay; F, female; IB, immunoblot; IIF, indirect immunofluorescence; IP, immunoprecipitation; KFA, keratinocyte footprint assay; Ln‐332, laminin‐332; M, male; na, not available; Neg, negative; np, not performed; SSS, salt‐split skin. IP was performed with radiolabelled human keratinocyte extract.34 IB was performed for all sera on extract of cultured keratinocytes, and for sera of patients 7, 9 and 16 also on human keratinocyte matrix proteins. ELISA for anti‐Col7 was performed with a kit from MBL (Nagoya, Japan) and ELISA for anti‐Ln‐332 was performed on affinity‐purified Ln‐332 as described previously.34 ^a–cDescribed previously by ^aTerra et al.;34 ^bvan den Bos et al.38 and ^cJonkman et al.37