Figure 2.

Alignment of protein sequences of EhSNX1, EhSNX2, and HsSNX3. Protein sequences were aligned using clustalw algorithm (http://clustalw.ddbj.nig.ac.jp). Phox homology domain sequences were predicted by HMMER (Meng & Ji, 2013) and are shown with yellow background. The numbers by three amino acid sequences (EhSNX1, EhSNX2, and HsSNX3) correspond to the a.a. positions of each protein, whereas those on top of the sequences (Arg69, Ser72, Lys95, Ile109, and Arg118) correspond to the positions of the residues in HsSNX3 (Lenoir et al., 2018). Ser72 was previously shown to be phosphorylated and marked with a blue rectangle, whereas the other residues implicated for PI3P binding are marked with red rectangles. Important residues for binding to Vps26 and Vps35 are shown with blue and pink background, respectively