Figure 3.

Lipid‐binding specificity of EhSNX1 and EhSNX2. (a) Verification of the expression of EhSNX1 and EhSNX2 in HA‐EhSNX1‐ and HA‐EhSNX2‐expressing transformants by immunoblotting using anti‐HA antibody. Approximately 20 μg of total lysates from these strains were loaded. Cysteine synthase 1 was detected by anti‐CS1 antiserum as a loading control. (b) Lipid‐binding specificity of EhSNX1 and EhSNX2 observed by lipid overlay assay. A panel of PIPs and phospholipids spotted on nitrocellulose membrane was incubated with total lysates from HA‐EhSNX1‐ and HA‐EhSNX2‐expressing transformants. The membranes were reacted with anti‐HA antibody. LPA, lysophosphatidic acid; LPC, lysophosphocholine; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; S1P, sphingosine‐1‐phosphate