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ITC measurements of BPPSA titrated with NQO1 WT or the NQO1 P187S variant. The dissociation constants for the binding of BPPSA to (A) NQO1 WT and (B) NQO1 P187S could be determined to be 7.95 ± 0.80 and 20.0 ± 0.41 μm, respectively. The experiments were performed in triplicates.
