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. 2019 Jul 26;287(3):465–482. doi: 10.1111/febs.14989

Figure 5.

Figure 5

Effect of glutamine and 2‐OG on PII‐mediated NAGKs activation. (A) Glutamine‐dependent activation of arginine‐inhibited Ppa NAGK or arginine‐inhibited Cr NAGK by Ppa PII, as indicated. (B) Effect of 2‐OG on Ppa PII and Cr PII proteins in the presence of 5‐mm glutamine on activation of arginine‐inhibited Ppa NAGK, as indicated. The assays were performed in presence of 0.5‐mm arginine for Ppa NAGK or 0.12‐mm arginine for Cr NAGK. Data were fitted using a graphpad prism, yielding an EC 50 for Gln and an IC 50 for 2‐OG. SD as indicated by error bars, represents triplicate independent measurements.