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. 2020 Jan 13;98(2):138–151. doi: 10.1111/imcb.12309

Figure 1.

Figure 1

Increased production of interleukin‐8 (IL‐8) by chronic myeloid leukemia (CML) with chronic and acute infection of mycoplasma. Noninfected K562 cells were treated with mycoplasma‐containing culture supernatant for 1, 3, 5 and 7 days (myco tx). These acutely infected cultures were compared with noninfected (NT) and chronically infected CML cultures (L). (a) Cell culture supernatants were tested for presence of mycoplasma via PCR. DNA bands were visualized via UV transillumination (Bio‐Rad imager and Syngene Genesnap software) of SYBR safe‐stained agarose gel. (b) Mycoplasma‐infected K562 cells were seeded at 1 million cells mL–1 and incubated overnight. Culture supernatants were tested for presence of IL‐8, IL‐6, tumor necrosis factor‐α (TNF‐α) and IL‐10 using ELISA. Results shown are mean ± s.e.m. of three independent experiments (donors). See Supplementary figure 1 for individual replicate experiments. Statistical significance was determined using repeated measures one‐way ANOVA followed by Tukey's test. ***P < 0.001. L, CML cells that were long‐term mycoplasma infected because of tissue culture procedures; n.d., nondetectable; ns, nonsignificant; NT, nontreated CML cells that were mycoplasma free.