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. 2020 Jan 13;98(2):138–151. doi: 10.1111/imcb.12309

Figure 2.

Figure 2

Macrophage (Mϕ) attenuated natural killer (NK) cell cytotoxicity against mycoplasma‐infected chronic myeloid leukemia (CML). (a) Experimental setup of mono, duo and triocultures with Mϕ, NK and CML cells (cell density ratios in parentheses). Cells were cocultured for 24 h prior to measurement of CML survival based on flow cytometry. Chronically infected cultures were annotated myco+, whereas noninfected cultures were annotated myco−. (b) Representative flow cytometry plots showing carboxyfluorescein succinimidyl ester‐stained cancer cells and % live cells based on negative staining for fixable viability dye. (c) CML survival measured under myco+ and myco− conditions, upon coculture with Mϕ, NK cells and normalized to cancer‐alone control. Results shown are mean ± s.e.m. of five independent experiments (donors). See Supplementary figure 4 for individual replicate experiments. Statistical significance was determined using repeated measures one‐way ANOVA followed by Fisher's LSD test. **P < 0.01. C, CML alone; MC, Mϕ + CML; MNC, Mϕ + NK + CML; NC, NK + CML; ns, nonsignificant.