Figure 4.

p53S Regulates CAFs Properties through Stat3 pathway. (A) GSEA plot indicating significant IL6-JAK-Stat3-activated signatures in p53^S/S MEFs. (B) Western blotting analysis of α-SMA, total-Stat3, p-Stat3, total-p53, p-p53 expression in WT, p53^-/-, p53^S/S MEFs p53^S/S MEFs treated with Stattic and p53R172H MEFs. (C) WT, p53^-/-, p53^S/S MEFs and p53^S/S MEFs treated with Stattic were subjected to a collagen gel contraction assay in duplicate and imaged after 24 hrs. Error bars represent SD (n=3), *p<0.05. (D) Transwell migration assay of H1299 cells following alone or co-culture with conditional media from WT, p53^-/-and p53^S/S MEFs and p53^S/S MEF treated with Stattic after 24 hrs. Average migration ±SEM from three independent experiments. Transwell invasion assay of H1299 cells following alone or co-culture with conditional media from WT, p53^-/-and p53^S/S MEFs and p53^S/S MEF treated with Stattic after 24 hrs. Average invasion ±SEM from three independent experiments. **p<0.005. (E) qRT-PCR analysis of α-SMA and FGF10 expression in WT, p53^-/-, p53^S/S MEFs and p53^S/S MEFs treated with Stattic. (F) ELISA analysis of CXCL12 expression in WT, p53^-/-, p53^S/S MEFs and p53^S/S MEFs treated with Stattic. *p<0.05; **p<0.005.