Figure 3.

Phylogenetic 16S rRNA gene tree showing 25 representative sequences of the OTUs of the four methanotrophic groups (in red) detected in Lacamas Lake natural waters and incubation experiments (labelled LL‐16S‐followed by a number) and their closest relatives. The most abundant OTUs in the incubations are indicated in bold. OTUs falling in the Methylobacter clade 1 cluster were not detected. The relative abundance of the LL‐16S‐ OTUs in each sample are shown in Table S8. The phylogenetic analysis was restricted to the sequence fragment (approximately 290 bp) obtained with the 16S rRNA amplicon sequencing analysis. Maximum likelihood estimation was performed using the General Time Reversible model.