Figure 2.

H₂S protects against HG-induced decrease in viability of HUVECs by activating PI3K/AKT/eNOS pathway. Cell viability was detected using by CCK-8 assay. HUVECs were treated with 33 mM glucose (HG concentration), or pretreated with 400 μM NaHS before HG treatment, or pretreated with 20 μM 740 Y-P before HG treatment, or co-treated with 10 μM LY294002 and 400 μM NaHS prior to HG treatment, or treated with 400 μM NaHS alone, or treated with 20 μM 740 Y-P alone, or treated with 10 μM LY294002 alone or treated with 33 mM mannitol alone. Experiments were repeated three times. Data are presented as the mean ± standard error of the mean. **p < 0.01 compared with the control group; ^##p < 0.01 compared with the group treated with HG; ^▲▲p < 0.01 compared with the group pretreated with NaHS before HG treatment. Con, control group; HG, high glucose (33 mM); YP, 740 Y-P; LY, LY294002; MAN, mannitol.