Figure 6:

IgG isolated from high fat diet (HFD)-fed mice raises blood pressure via FcγRIIB, and endothelial FcγRIIB mediates obesity-induced hypertension. Male B^−/− and B^−/−;FcγRIIB^−/− mice were fed a HFD for 12–14 weeks, and over the ensuing 4 weeks while remaining on HFD, they were i.p. injected with IgG (150μg/mouse, 2 times/week) isolated from wild-type mice fed a HFD. Body weight was determined (A), and systolic blood pressure (SBP) was measured by tail cuff (B). In studies of FcγRIIB in endothelium, male control floxed FcγRIIB mice (FcγRIIB^fl/fl) and mice lacking the receptor selectively in endothelial cells (FcγRIIB^ΔEC) were placed on control chow (Con) or high fat diet (HFD) for 12 weeks, body weight was determined (C), and SBP was measured (D). Values are mean±SEM. N=8–11 per group (A,B), and n=17–19 per group (C,D). *p<0.05, *** p<0.001, **** p<0.0001.