Figure 7:

ManNAc supplementation protects mice from obesity-induced hypertension. Male wild-type mice were placed on control chow (Con) or high fat diet (HFD) for 16 weeks, and during that time the HFD-fed mice received either regular drinking water or ManNAc-supplemented drinking water. Body weight was measured every 4 weeks (A). Plasma IgG was isolated, and its sialylation was evaluated by SNA-lectin blotting (B). Graph depicts the relative sialylation. Systolic blood pressure (SBP) was measured by tail cuff (C). Values are mean±SEM, n=8 per group. In A, comparisons with the Control group were made by two-way ANOVA and Tukey’s multiple comparison test. ** p<0.01, *** p<0.001, **** p<0.0001.