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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2019 Nov 20;86:102752. doi: 10.1016/j.dnarep.2019.102752

Figure 1.

Figure 1.

NEIL3-GD selectively unhooks the dA-AP cross-link in a splayed duplex. The 5’-32P-labeled cross-linked duplexes A and B were incubated with NEIL3 in HEPES (20 mM, pH 7.4) containing NaCl (100 mM), EDTA (1 mM), dithiothreitol (1 mM) and bovine serum albumin (100 μg/mL) at 37 °C for 2 h. Formamide loading buffer was added and the DNA in the samples resolved by electrophoresis on a denaturing 20% polyacrylamide gel. Following separation, the 32P-labeled oligonucleotides in the gel were visualized by phosphorimager analysis.