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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2019 Nov 20;86:102752. doi: 10.1016/j.dnarep.2019.102752

Figure 5.

Figure 5.

Fluorescence anisotropy experiments provide evidence that NEIL3-GD has the same affinity for leading and lagging strand ICL substrates. Solutions containing FAM-labeled AP-ICL substrate were titrated with the catalytically-inactive E3Q mutant of NEIL3-GD. The plot shows average ± S.D. for three independent measurements. The calculated Kd for the binding of NEIL3-GD E3Q to the leading strand ICL is 0.5±0.1 μM and that for the lagging strand ICL is 0.4 ± 0.2 μM. In the fork schematics, the blue line indicates the position of the ICL and the asterisk denotes the position of the FAM label.