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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Heart Rhythm. 2019 May 22;16(11):1720–1728. doi: 10.1016/j.hrthm.2019.05.015

Figure 3. β-catΔE3 interacts with TCF4.

Figure 3.

Total protein was extracted from isolated adult CTNNB1 E3+/+ (n=3 mice) and CTNNB1 E3−/− VMs (n=3 mice). Western blot analysis showing β-catΔE3 in CTNNB1 ES−/− VMs and abundant full-length β-catenin detected in CTNNB1 E3+/+ VMs, but not in CTNNB1 E3−/− VMs (A). TCF4 detected in both CTNNB1 E3+/+ and CTNNB1 E3−/− VMs (B). IP was performed on the same amount of protein extracted from isolated adult CTNNB1 E3+/+ and CTNNB1 E3−/− VMs by using a β-catenin antibody and Western blotting showing that this antibody pulls down β-catenin in CTNNB1 E3+/+ MV and β-catΔE3 with much less full-length β-catenin in CTNNB1 E3−/− MV (A). This antibody pulled down more TCF4 in CTNNB1 E3−/− than CTNNB1 E3+/+ MV (B). Student’s t-test showing that β-catΔE3 protein in CTNNB1 E3−/− VMs is significantly increased (*p<0.05) compared to full-length β-catenin in CTNNB1 E3+/+ VMs (C); there is no significant difference in TCF4 expression between CTNNB1 E3+/+ and CTNNB1 E3−/− VMs (C). Immunoprecipitated TCF4 relative to β-catenin is significantly increased (**p<0.01) in CTNNB1 E3−/− VMs compared to that CTNNB1 E3+/+ VMs (D).