Table 1.

miRNAs modulated by Activin‐A in osteoblasts, 4 hr and 1 day after the start of Activin‐A treatment. Intensities are indicated as AVG signal

	Mature miRNAs	A‐OBs (AVG signal)	NT‐OBs (AVG signal)	Mature miRNAs	A‐OBs (AVG signal)	NT‐OBs (AVG signal)
4 hr	>2‐Fold upregulated in A‐OBs			>2‐Fold downregulated in A‐OBs
	miR‐590‐3p	790.81	351.31	miR‐486‐3p	1,788.62	4,489.87
	miR‐142‐3p	386.24	178.37	miR‐19a	1,209.74	2,925.18
	miR‐18b	609.81	300.37	miR‐33a	122.18	1,942.43
				miR‐32	171.06	439.93
				miR‐150	142.06	358.49
	Detected only in A‐OBs			Not detected in A‐OBs
	miR‐432*	106.87		miR‐573		84.24
	miR‐337:9.1	88.12		miR‐483‐5p		76.06
	miR‐33b*	84.37		miR‐1263		75.37
	miR‐96	77.87		miR‐371‐3p		70.37
	miR‐592	76.87		miR‐186*		69.06
	miR‐744*	68.56		miR‐765		66.74
	miR‐338‐3p*	67.99		miR‐198		65.06
	miR‐517a, b	61.68		miR‐563		63.81
	miR‐20b	60.93		miR‐10b		62.18
	miR‐645	59.31		miR‐891a		59.18
	miR‐580	58.93
	miR‐744*	58.68
1 day	>2‐Fold upregulated in A‐OBs			>2‐Fold downregulated in A‐OBs
	miR‐486‐3p	4,929.56	2,055.87	miR‐193a‐3p	963.43	2,079.74
	miR‐100*	993.68	453.87	miR‐590‐3p	126.06	432.18
	miR‐150	390.12	184.31
	miR‐24‐1*	258.74	122.43
	Detected only in A‐OBs			Not detected in A‐OBs
	miR‐18b	203.18		miR‐220b		94.87
	miR‐217	107.74		let‐7f‐2*		74.37
	miR‐548o	83.43		miR‐431*		69.99
	miR‐589*	79.99		miR‐610		67.62
	miR‐548f	75.74		miR‐518e		66.06
	miR‐181d	71.93		miR‐632		65.74
	miR‐218‐2	66.56		miR‐520d‐5p		63.06
	miR‐1208	64.37
	miR‐921	64.31
	miR‐432*	63.56
	miR‐563	61.74
	miR‐559	57.43
	miR‐645	55.68
	miR‐517a,b	54.31
	miR‐1236	54.06

Abbreviations: A‐OBs, Activin‐A treated osteoblasts; miRNAs, microRNAs; NT‐OBs, non‐treated osteoblasts.