Figure 4.
Down-regulated SBF2-AS1 and MBNL3 expression while up-regulated miR-302a expression in NCI-H1299 parental strain and resistant strain after transfection. (a) Expression of SBF2-AS1 in NCI-H1299 and NCI-H299R cells after transfection. (b) Expression of miR-302a in NCI-H1299 and NCI-H299R cells after transfection. (c) Expression of MBNL3 mRNA in NCI-H1299 and NCI-H299R cells after transfection. (d&e) Protein expression of MBNL3 in NCI-H1299 and NCI-H299R cells after transfected with si-SBF2-AS1 and miR-302a mimics verified by western blot assay. N = 3, * P < 0.05 vs. the si-NC group, # P < 0.05 vs. the mimics NC group. Measurement data were depicted as mean ± standard deviation, and comparisons between two groups were conducted by independent sample t-test, comparisons among multiple groups were assessed by one-way analysis of variance followed by LSD-t test.