Table 2. Characteristics of the primers used for PCRs.
Primer | Sequence (5´- 3´) | Fragment size (bp) | Temperature annealing (°C) | Reference |
---|---|---|---|---|
18SFr | CGAGCAATAACAGGTCTGTG | 200 | 50°C | Mauri et al 2011 |
18SRw | GGCAGGGACTTAATCAA | |||
HPNF3 | CATTAGCATGGAATAATAAAACACGAC | 600 | 55°C | Catanese et al 2018 |
HPNR3 | GCGACGGCTATTTAGATGGCTGA | |||
HpF | GGTACGGAGAATCCGGGGTT | 1409 | 55 °C | This study |
HpR | ACTTGTCCTTCCTCTAATAATAAGG | |||
HpF3 | GCGGGCTTAGTTCAGGGG | 165 | 60 °C | This study |
HpR3 | AAAACCAACAAAGGCCCGAA |