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. 2019 Nov 4;31(3):260–271. doi: 10.1097/CAD.0000000000000865

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Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Fig. 2 — CACNA2D1 and miR-107 are inversely correlated in LSCC cell lines. (a and b) Transfection efficiency among the following five groups: TU212 and TU686 cells transfected with blank control, agomiR NC, agomiR-107, antagomiR NC, or antagomiR-107, and the expression of miR-107 was significantly increased or decreased, respectively (compared with the control group *P < 0.05). (c and d) qPCR analysis of relative expression level of CACNA2D1 mRNA after transfection of TU212 and TU686 cells (compared with the control group *P < 0.05). (e and f) Western blot analysis of relative expression of α2δ1 protein in transfected TU212 and TU686 cells (compared with the control group, *P < 0.05). LSCC, laryngeal squamous cell carcinoma; miR-107, microRNA-107; NC, negative control.