Fig. 3.

CACNA2D1 is a direct downstream target of miR-107. (a) Wild type and mutant of the 3′-UTR of CACNA2D1 mRNA and miR-107 binding site (site A: 202-209). (b) Luciferase activity controlled by the 3′-UTR of CACNA2D1 was inhibited by ectopic expression of miR-107 at site A. TU686 cells were co-transfected with wild-type or mutant CACNA2D1 3′-UTR and agomiR-107, antagomiR-107 and the corresponding negative controls. Renilla and firefly luciferase activities were measured using a dual-luciferase reporter assay system. The ratio of renilla luciferase activity to firefly luciferase activity was normalized, and the data are expressed as mean ± SEM (compared with the control group, *P < 0.05). (c) Wild type and mutant of the 3′-UTR of CACNA2D1 mRNA and miR-107 binding site (site B: 902-908). (d) Luciferase activity controlled by the 3′-UTR of CACNA2D1 was inhibited by ectopic expression of miR-107 at site B. TU686 cells were co-transfected with wild-type or mutant CACNA2D1 3′-UTR and agomiR-107, antagomiR-107, and the corresponding negative controls. The dual-luciferase reporter gene system indicates that miR-107 can bind to the site B of CACNA2D1 3′-UTR and inhibit the luciferase activity (compared with the control group, *P < 0.05). miR-107, microRNA-107