Skip to main content
. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Nat Metab. 2019 Oct 28;1(11):1089–1100. doi: 10.1038/s42255-019-0127-7

Figure 1: Global IDOL knockout mice are protected from diet-induced metabolic dysfunction.

Figure 1:

(a) Growth curve for wild-type (WT) vs. Idol knockout (KO) mice fed western diet from 5–6 weeks of age. The mean masses are shown +/− the standard error of the mean (SEM); n=12 WT, n=9 KO mice *p<0.05 by repeat measures two-way ANOVA. (b) Adiposity reported as body fat percentage +/− the SEM measured by MRI after 15 weeks of western diet feeding; n=5 WT, n=7 KO mice ****p<0.0001 by 2-tailed t-test. (c) H&E-stained sections of liver and epididymal white adipose tissue (eWAT) depots from mice maintained on western diet for 15 weeks; these images are representative of tissues analyzed from both the UCLA-produced and AstraZeneca-produced lines. (d) Intraperitoneal glucose tolerance test (1 mg/kg) administered after 9 weeks of western diet feeding. The mean blood glucose levels are shown +/− the SEM; n=10 WT, n=9 KO mice *p<0.05 by 2-tailed t-test of the area under the curve (AUC) (e) Intraperitoneal insulin tolerance test (1 U/kg) administered after 14 weeks of western diet feeding. The mean blood glucose levels are shown +/− SEM; n=7 WT, n=6 KO mice *p<0.05 by 2-tailed t-test of the area under the curve (AUC). (f-h) Single-housed IDOL KO mice consume less food than WT littermates, protecting them from diet-induced adipose expansion; n=14 WT, n=12 KO mice **p<0.01, ****p<0.0001 WT vs. KO by repeat measures ANOVA. (f) The mean food consumed per mouse is labeled +/− SEM. (g) The mean mass gained per mouse after being placed in single housing +/− SEM. (h) Adiposity reported as body fat percentage +/− the SEM measured by MRI. The precise n-number, p-value, and details of the statistical testing are provided in the source data file.