Extended Data Figure 7: Neuron-specific virogenetic deletion of IDOL from individual hypothalamic nuclei is insufficient to protect against diet-induced obesity.

(a-c) Adeno-associated virus (AAV) expressing either GFP-Cre or GFP regulated by the Synapsin I (SynI) promoter were injected into the arcuate nucleus (ARC: panels a-c) or the paraventricular nucleus of the hypothalamus (PVH: panels d-f). (a-c) Deletion of IDOL from neurons in the ARC had no effect on body mass or food intake for mice fed a western diet for 12 weeks; n=8 mice injected with AAV-expressing GFP (GFP^ARC-SynI), n=8 mice injected with AAV expressing Cre-GFP (Cre^ARC-SynI). (a) An image of the ARC showing GFP-positive cells to demonstrate successful infection of neurons; the image is representative of the sixteen mice injected in the ARC study. (b) Growth curve showing the mean mass of the mice from each treatment group +/− one standard deviation. (c) The mean cumulative mass of food consumed per mouse +/− one standard deviation. (d-f) Deletion of IDOL from neurons in the PVH had no effect on body mass or food intake for mice fed a western diet for 12 weeks; n=8 mice injected with AAV expressing GFP (GFP^PVH-SynI), n=9 mice injected with AAV expressing Cre-GFP (Cre^PVH-SynI). (d) An image of the PVH showing GFP-positive cells to demonstrate successful infection of neurons; the image is representative of the seventeen mice injected in the PVH experiment. (e) Growth curve showing the mean mass of the mice from each treatment group +/− one standard deviation. (f) The mean cumulative mass of food consumed per mouse +/− one standard deviation.