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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Nat Metab. 2019 Oct 28;1(11):1089–1100. doi: 10.1038/s42255-019-0127-7

Figure 6: Conditional deletion of Idol from neurons drives the metabolic protection seen in global IDOL KO mice.

Figure 6:

(a) Growth curve showing the mean mass +/− SEM and (b) Adiposity reported as body fat percentage +/− SEM measured by MRI for three cohorts of neuron-specific IDOL conditional knockout mice fed a western diet starting at 5–6 weeks old; n=18 per genotype *p<0.05, **p<0.01, ***p<0.001 ****p<0.0001 Idolf/f vs. Idolf/f CreSynI mice by repeat measures ANOVA. (c) Neuron-specific deletion of IDOL reduces food intake. The mean cumulative food consumption is shown +/− SEM; n=7 per genotype *p<0.05, ***p<0.001 Idolf/f vs. Idolf/f CreSynI mice by repeat measures ANOVA. (d) Increased phosphorylation of hormone sensitive lipase in the subcutaneous white adipose tissue of neuron-specific conditional IDOL KO mice. Each lane was loaded with a protein lysate from independent mice. This blot is representative of two independent repeats. (e,f) Global IDOL KO mice are protected from diet-induced mass gain and adiposity when group-housed at thermoneutrality; n=6 WT, n=8 IDOL KO mice (e) Growth curve showing the mean mass gained +/− SEM. (f) Adiposity reported as body fat percentage +/− SEM measured by MRI for adiposity. *p<0.05, ***p<0.001, ****p<0.0001 WT vs. KO by repeat-measures ANOVA. The precise n-number, p-value, and details of the statistical testing are provided in the source data file.