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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Nat Metab. 2019 Oct 28;1(11):1089–1100. doi: 10.1038/s42255-019-0127-7

Extended Data Figure 1. Metabolic phenotype of IDOL-deficient mice.

Extended Data Figure 1.

(a,b) 18-month-old IDOL KO mice are protected against age-induced adiposity. (a) Mean mass and standard error of the mean; n=9 WT, n=10 KO mice, ***p<0.001, ****p<0.0001 vs. WT by two-tailed t-test. (b) Mean body fat percentage +/− the standard error of the mean; n=9 WT, n=10 KO mice, ***p<0.001, ****p<0.0001 vs. WT 2-tailed t-test. (c) Growth curve for littermate male mice fed a 60% kcal high fat diet (HFD) starting when the mice were 6 weeks old. The mean values are shown +/− the standard error of the mean; n=5 WT and n=6 KO mice *p<0.05 by repeat measures ANOVA. (d) Growth curve for littermate male IDOL(AZ)f/f mice with or without whole-body Rosa26 Cre fed a low-fat diet from 8 weeks of age. The mean mass is shown +/− SEM; n=10 mice per group. (e) Growth curve for littermate male IDOL(AZ)f/f mice with or without whole-body Rosa26 Cre fed a high fat high cholesterol diet from 8-weeks of age; n=8 Idol(AZ)f/f mice n=9 CreR26+Idol(AZ)f/f mice; *p<0.05 by repeat measures ANOVA (f) Unchanged lean body mass in male IDOL knockout mice administered test diets despite adiposity changes measured by MRI. Mean lean mass is shown +/− SEM; n=9 WT and n=10 KO mice fed Chow, n=5 WT and n=7 mice fed western diet, n=5 WT and n=6 KO mice fed the 60% HFD, p-values calculated by two-way ANOVA with Sidak post hoc tests. (g) Body length measured from nose to anus at 20 weeks of age for male mice fed either a low-fat diet (LFD) or a high-fat high-cholesterol diet (HFHC) for 12 weeks. The mean values are shown +/− the standard error of the mean. Low-fat diet: n=10 mice per genotype. High Fat High Cholesterol diet: n=8 Idol(AZ)f/f mice and n=9 CreR26+Idol(AZ)f/f mice. P-values calculated by repeat measures ANOVA. (h) Mean body temperature +/− SEM measured rectally in response to fasting in mice fed a high-fat high-cholesterol diet; n=8 WT and n=9 IDOL KO mice. The precise n-number, p-value, and details of all statistical testing are provided in the source data file.