Extended Data Figure 2: Glucose and insulin tolerance tests conducted on Idol^f/f and conditional knockouts with tissue-specific deletion of IDOL.

(a-b) Mean blood glucose levels +/− SEM for liver-specific conditional IDOL KO mice challenged with an oral glucose tolerance test (2g/kg) at 22 weeks of age fed either (a) the low-fat diet; n=12 mice per genotype or (b) the high-fat high-cholesterol diet for 16 weeks; n=11 mice per genotype. (c-j) Mean blood glucose levels +/− SEM for male mice fed a western diet challenged with an intraperitoneal glucose tolerance test (1 g/kg, shown on the left) after six weeks on diet and an intraperitoneal insulin tolerance test (1 U/kg, shown on the right) after ten weeks on diet. (c,d) Adipose-specific conditional IDOL KO; n=11 Idol^f/f, n=9 Cre^AdipoQIdol^f/f mice. (e,f) Endothelium-specific conditional IDOL KO; n=13 Idol^f/f, n=10 Cre^Cdh5Idol^f/f mice. (g,h) Intestine-specific conditional IDOL KO; n=8 Idol^f/f, n=4 Cre^VilIdol^f/f mice. (i,j) Muscle-specific conditional IDOL KO; n=10 mice per genotype for the GTT and n=10 Idol^f/f, n=9 Cre^MckIdol^f/f mice for the ITT.