Extended Data Figure 3: Adipose-specific transgenic IDOL mice are not protected from diet-induced obesity.
(A) Ablation of VLDLR protein levels in the subcutaneous inguinal white adipose tissue of the aP2-IDOL transgenic mice. This blot is representative of many independent experiments conducted by two independent researchers. (B) Reduced VLDLR protein in the interscapular brown adipose tissue of the aP2-IDOL transgenic mice. This blot is representative of many independent experiments conducted by two independent researchers. (c) Growth curve for WT and aP2-IDOL transgenic mice fed a western diet from 5 weeks of age showing the mean mass +/− SEM; n=13 WT and n=9 aP2-IDOL transgenic mice (d) Mean body fat percentage measured by MRI +/− SEM for n=13 WT and n-9 aP2-IDOL transgenic mice after 12 weeks of western diet feeding. (e) Intraperitoneal glucose tolerance test (1g/kg) administered after six weeks of western diet feeding; n=13 WT and n-9 aP2-IDOL transgenic mice. (f) Intraperitoneal insulin tolerance test (1U/kg) administered after ten weeks of western diet feeding n=13 WT and n-9 aP2-IDOL transgenic mice.
