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. Author manuscript; available in PMC: 2020 Sep 18.
Published in final edited form as: ACS Chem Neurosci. 2019 Aug 19;10(9):4018–4030. doi: 10.1021/acschemneuro.9b00271

Figure 3.

Figure 3.

Differentiated SH-SY5Y cells were a valid neuronal model. (A) Western blotting on a whole-cell lysate. Analysis was performed with antibodies against sEH (EPHX2), p38β kinase, and β-actin (loading control). Optical densities were normalized to β-actin. (B) Treatment with various concentrations of TPPU (10 to 1000 nM) for 24 h significantly decreased cellular sEH activities in SH-SY5Y cells. Analysis was performed with a sEH enzyme assay using a radiolabeled substrate t-DPPO. Data were the mean of three independent experiments with ± SEM (n = 3), *p < 0.05, ***p < 0.001.