Figure 2.
Safety evaluation of OVH in vivo. (A) Murine lethal challenge model and timeline of challenge and follow-up. (B) Survival over time for mice intracerebrally (i.c.) injected with indicated dosages of viruses or PBS in 10 μl. (C) The replication efficiency of KOS, dICP0, OVN and OVH in U-2 OS cells exposed to different doses of ganciclovir (0 ng/mL~1000 ng/mL, MOI = 0.05 PFU/cell). (D) Timeline of the toxicity study. Complete blood count (CBC) analysis and immunohistochemistry (IHC) analysis were performed at indicated time points. (E-J) Mice intravenously injected with a single dose of OVH (5 × 107 PFU) were bled on day 1 and day 30 post-injection, and WBC, PLT, lymphocytes, RBC and neutrophil granulocytes were measured, n = 6 BALB/c mice per group. H&E staining of representative tissue sections from vehicle- and OVH-injected mice on day 30 following virus injection (E). H&E images were obtained using a microscope (OLYMPLUS), with a 20 × objective. (K) The body weight of the treated mice was monitored over a 30-day period. (l) Timeline of biochemistry analysis after mice were intravenously challenged with 5 × 107 PFU OVH or PBS. (M-T) ALB, TP, CREA, UA, UREA, and T-Bil levels as well as AST and ALT activity were measured, n = 10 BALB/c mice per group. Arrows indicate corresponding time points. Data are shown as means ± SEM. **P < .01, ns, not significant by unpaired two-tailed Student’s t tests for E, F, G, I, J or repeated measure ANOVA for K, M-T.