Figure 6.

Cryopreservation has a negligible effect on maturation kinetics and the functionality of in vitro generated TA-specific T-cells. (a) Relative cell numbers at day 14 of co-cultures (day at which cord blood progenitors were transduced) from fresh and cryopreserved (frozen) HSPC from cord blood (n = 6), healthy donors (n = 6), patients in remission after chemotherapy (n = 7) and AML patients at diagnosis (n = 6). Individual fresh and paired frozen samples are shown. (b) Percentage specific lysis determined via 4-h ⁵¹chromium release assay after co-culture of T-cells with T2 cells pulsed with relevant WT1 or irrelevant influenza (INF) peptide (10 µg/ml), HL-60-A2 (HLA-A2⁺ WT1⁺), THP-1 (HLA-A2⁺ WT1⁺) or JY (HLA-A2⁺ WT1⁻) cells. Effector/target ratio 5/1. T-cells generated from fresh and cryopreserved (frozen) HSPC (n = 11). Results from different sample groups (healthy donors, patients in remission and AML patients at diagnosis) were pooled. Mean and s.d. are shown. Wilcoxon matched-pairs signed-rank test was used to assess statistical significance. Differences were not significant.