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. 2020 Feb 14;9(1):1725355. doi: 10.1080/2162402X.2020.1725355

Figure 7.

Figure 7.

S100A4 induced β-catenin signaling in collagen I cultured Huh-7 cells was RAGE dependent. (a) The expression of RAGE in HCC cells was upregulated after collagen treatment. Huh-7 cells were cultured with or without collagen I and incubated with S100A4 (200 ng/ml) for different time as indicated. Levels of RAGE in Huh-7 cells were determined by western blot. (b) Huh-7 cells were cultured as (a) described, levels of p-β-catenin in Huh-7 cells were determined by western blot analysis. (c) Huh-7 cells were cultured as (a) described, RAGE inhibitor FPS-ZM1 (75 nmol/μl) was added or not, levels of p-β-catenin were determined by western blot analysis. (d) The expression of OCT-4, SOX2, CD44 and Nanog in Huh-7 cells were detected by real-time PCR. * P < .05, ** P < .01, *** P < .001.