Figure 2.

Intracellular fate of AM-PEG NSs. The Cy5.5-labeled AM-PEG NSs enter MCF-7 cells through: a) Macropinocytosis and b) Caveolin-dependent endocytosis. Cytoplasmic fluorescence measured by FCM of cells pretreated with: c) micropinocytosis pathway inhibitor (Rot) and Caveolin-dependent pathway inhibitor (Nys), and d) Clathrin-dependent pathway inhibitors (Cho and Suc) for 2 h. After endocytosis, the AM-PEG NSs are transported by: e) early endosomes, f) late endosomes, and finally into: g) lysosome. h) Schematic representation of how FITC-labeled AM-PEG NSs enter and travel in the MCF-7 cells (scale bars: 10 μm).