Figure 3.

Photo-induced benefits and in vitro combined cancer therapy. a) CLSM images of MCF-7 3D multicellular tumor spheroids after incubation with AM-PEG/DOX NSs for 3 h without (W/O) or with pre-treatment of 808-nm laser irradiation. Z-stack images were obtained from the top toward the tumor spheroid equatorial plane at 10-μm intervals. b) Fluorescence intensity measured from the equatorial plane of tumor spheroid in the W/O laser group. c) Fluorescence intensity measured from the equatorial plane of tumor spheroid in the with laser group. d) FCM histogram profiles of cellular DOX fluorescence intensity in MCF-7 cells W/O or with laser irradiation. e) Thermal images of 96-well plate during laser irradiation at final stage. f) Cell viability of MCF-7 cells with different treatments.