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. Author manuscript; available in PMC: 2020 Feb 18.
Published in final edited form as: Science. 2019 Jun 21;364(6446):1166–1169. doi: 10.1126/science.aaw1143

Fig. 2. Scope of biocatalytic radical cyclization.

Fig. 2.

Reaction conditions: GluER-T36A (0.5 mol %), NADP+ (1 mol %), GDH-105 (0.2 mg/mg starting material), glucose (6 equiv.), KPi (pH = 8.0, 100 mM, with 10% glycerol), substrate (1 equiv). 50W Cyan LED. Average temperature 35 °C. a Results obtained using NostocER and favors the opposite product enantiomer. b 12% formation of the hydrodehalogenated product. c 1 mol % enzyme loading (18 hours). d 2 mol % enzyme loading (18 hours). e 4 mol % enzyme loading (72 hours).