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Time dependent cytotoxicity of hRS7, control ADC and SG after 24, 48, and 72 h exposure. Down right and up left quadrants show single positive events for FL1-H (ANNEXIN V-FITC, apoptosis) and FL2-H (Propidium Iodide, necrosis), respectively. Up right quadrant shows double positive events for FL1-H and FL2-H, respectively. Double positive events stand for tardive apoptosis. Maximal cytotoxicity of SG was demonstrated after 72 h exposure.
