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. 2020 Feb 12;11:54. doi: 10.3389/fimmu.2020.00054

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Copyright © 2020 Bulati, Miceli, Gallo, Amico, Carcione, Pampalone and Conaldi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of immunomodulatory factors. Both protein (A,B) and gene (C,D) expression were assayed after 24 or 48 h of cultures in CM and cells, respectively. (A) Protein expression of immunomodulator factor in hAMSCs cultured for both 24 and 48 h. (B) Protein expression of immunomodulator factor in hAMSCs cultured for 48 h and treated with poly (I:C) or LPS. (C) Gene expression of immunomodulator factor in hAMSCs cultured for 24 h and treated with poly (I:C) or LPS. (D) Gene expression of immunomodulator factor in hAMSCs cultured for 48 h and treated with poly (I:C) or LPS. Transcript levels were normalized to those of GAPDH, and expressed as fold change vs. untreated cells (control). Data are means ± SD. ^*p < 0.05 vs. control.