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. 2020 Feb 12;11:54. doi: 10.3389/fimmu.2020.00054

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Copyright © 2020 Bulati, Miceli, Gallo, Amico, Carcione, Pampalone and Conaldi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentially expressed miRNAs in EXO derived from hAMSCs. (A) Hierarchical clustering based on Z ratios (calculated as described in the Materials and Methods section) of miRNA expression levels in EXO derived from hAMSCs treated with poly (I:C), LPS, and IFNγ. (B) Upregulated miRNAs in EXO derived from hAMSCs treated with IFNγ. (C) Downregulated miRNAs in EXO derived from hAMSCs treated with IFNγ. (D) Volcano plot analysis of deregulated miRNAs in EXO derived from hAMSCs treated with IFNγ (p ≤ 0.05 and fold change ≥ 1.5). miRNA levels were normalized to those of U6, and expressed as fold change vs. untreated cells (control). Data are means ± SD.