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. 2020 Feb 12;11:145. doi: 10.3389/fimmu.2020.00145

Figure 2.

Figure 2

PP13 does not influence the functionality of neutrophils. (A) Spontaneous or induced NET formation after 2 h stimulation of neutrophils with or without PP13 (3 μg/ml) and 1 h ± PMA or A23187 (A23) was quantified by immunostaining of neutrophils (n = 4). The quantification analysis was performed by NETQUANT. (B) NE activity assay performed on supernatants from neutrophils cultured in the presence (n = 4) or absence (n = 5) of PP13 (3 μg/ml) or control Gal (n = 5) for 24 h. (C) Phagocytosis of FITC-dextran measured with flow cytometry in neutrophils cultured in the presence or absence of PP13 (3 μg/ml) for 3 days and exposed to FITC-dextran for 60 min (n = 5). (D) Production of ROS by neutrophils cultured with PP13 (3 μg/ml) or a control Galectin for 24 h (n = 6). (E) ROS production by neutrophils stimulated with PP13 (3 μg/ml) (triangles) for 30 min with (triangles) or without (circles) heat-killed E. coli or S. aureus (n = 5). All analysis was performed with One-way ANOVA unpaired test, ***p < 0.001.