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. 2020 Feb 12;11:13. doi: 10.3389/fendo.2020.00013

Figure 4.

Figure 4

Phosphorylation of c-Jun in wildtype (WT) and IKKβ-deficient MC3T3–E1 cells. (A,B) Representative data of phospho-c-Jun (A) at 60 min after β-GP and AA stimulation, and mean fluorescence intensity (MFI) of phospho-c-Jun (B) at 0, 60, and 120 min after β-GP and AA treatment, compared between WT and IKKβ-deficient cells (n = 5). Statistics by one-way ANOVA. (C) Western blot analysis for phospho-c-Jun proteins in WT and IKKβ-deficient MC3T3–E1 cells after the treatment described in B. Images were representatives of three independent experiments. (D) Representative immunofluorescence images of phospho-c-Jun in WT and IKKβ-deficient MC3T3–E1 cells after 0, 60 min treatment with β-GP and AA. Scale bars: 50 μm. Error bars represent ± SEM. ***P < 0.001, ns = not significant.