Figure 5.

Deletion of JNK1 or JNK2 in IKKβ-deficient MC3T3–E1 cells. (A,B) Schematic diagram showing the adjacently placed sgRNAs designed to target mouse Mapk8 or Mapk9, which are located on different murine chromosomes. PAM motifs were shown in red color. (C) Genomic edition results of representative clones of Mapk8^−/− or Mapk9^−/− validated by PCR and agarose electrophoresis in Ikbkb^−/− clone 26. Control (CTR) referred to Mapk8 or Mapk9 untargeted Ikbkb^−/− clone 26 and H₂O was used as negative control. (D) MC3T3–E1 wildtype (WT), Ikbkb^−/− clone 26, Ikbkb^−/− Mapk8^−/− clone C1, and Ikbkb^−/− Mapk9^−/− clone D7 were treated with β-GP and AA for indicated time points. Western blot analysis was performed with the indicated antibodies. Images were representatives of three independent experiments.