Figure 7.

Influence of JNK1 or JNK2 deficiency on Ikbkb^−/− cells at transcriptomics level. (A) Volcano plot shows mRNAs of DEGs between control groups and osteogenic induction groups involving three types of cells namely Ikbkb^−/−, Ikbkb^−/− Mapk8^−/−, and Ikbkb^−/− Mapk9^−/− cells. The horizontal axis represents the fold changes between control groups and osteogenic induction groups. The vertical axis represents the q-value of the difference. Representative DEGs were pointed out by arrows to illustrate the gene expression changed by JNK1 or JNK2 deficiency during the response of IKKβ-deficient cells to osteogenic induction at day 4. (B) FPKMs of mRNAs of representative DEGs analyzed by RNA-seq. (C) DEGs of Ikbkb^−/− Mapk8^−/− cells compared with Ikbkb^−/− cells and DEGs of Ikbkb^−/− Mapk9^−/− cells compared with Ikbkb^−/− cells after 4 days of induction were classified into two categories in Venn diagram, the overlapping DEGs could be regulated by both JNK1 and JNK2. (D) Clustering results of the overlapping DEGs described in C. Expression profiles of these overlapping DEGs in Ikbkb^−/− Mapk8^−/− cells and Ikbkb^−/− Mapk9^−/− cells after induction were very similar in pattern. (E,F) KEGG pathway analysis and GO analysis of overlapping DEGs described in C. (E) The top 15 enrichment scores (–log10, P-value) of KEGG pathways, term candidate gene numbers were listed on the right. (F) GO analysis with top 20 representative most significantly enriched (–log10, P-value) GO terms of the overlapping DEGs covering biological processes and cellular components domains. Error bars represent ± SEM.